ON-TARGETplus Mouse Prkaa1 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate HiPerFect Transfection Reagent to diluted siRNA for 10–15 min at RT. Incubate the cells with the transfection complexes under their normal growth conditions for 24 h

Protocol tips
Incubate HiPerFect Transfection Reagent to diluted siRNA for 10–15 min at RT. Incubate the cells with the transfection complexes under their normal growth conditions for 24 h

Publication protocol

RAW264.7 macrophages were transfected with ON-TARGETplus Mouse Prkaa1 siRNA (a mixture of four Prkaa1 siRNAs) (Dharmacon/Thermo Scientific, Lafayette, CO) or ON-TARGETplus Non-targeting Pool (a mixture of four negative control siRNAs) (Dharmacon/Thermo Scientific) utilizing HiPerFect transfection reagent according to manufacture’s recommended protocols (Qiagen, Valencia, CA). The medium was changed 24 h after transfection. The transfection efficiency was determined by Western blot and densitometric analysis of bands 4 days after siRNA transfections.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Mouse Prkaa1 siRNA below.

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