Mm_Hdac5_2 FlexiTube siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	MmHDAC5_2 S-GCCUCGGAACCCAACUUAATT AS UUAAGUUGGGUUCCGAGGCCG MmHDAC5_5 S-GGGCAAGAUCCUUACCAAATT AS-UUUGGUAAGGAUCUUGCCCAG HsHDAC5_1 SACGACACGUUCAUGCUAAATT AS-UUUAGCAUGAACGUGUCGUAG HsHDAC5_4 S-CGGGUUUGAUGCUGUUGAATT AS-UUCAACAGCAUCAAACCCGGC siRNA concentration-120 pmol

Protocol tips
MmHDAC5_2 S-GCCUCGGAACCCAACUUAATT AS UUAAGUUGGGUUCCGAGGCCG MmHDAC5_5 S-GGGCAAGAUCCUUACCAAATT AS-UUUGGUAAGGAUCUUGCCCAG HsHDAC5_1 SACGACACGUUCAUGCUAAATT AS-UUUAGCAUGAACGUGUCGUAG HsHDAC5_4 S-CGGGUUUGAUGCUGUUGAATT AS-UUCAACAGCAUCAAACCCGGC siRNA concentration-120 pmol

Publication protocol

Lipopolysaccharide-free plasmid DNA (6 μg) and siRNA (120 pmol; Qiagen, Hilden, Germany) was transferred by electroporation as described previously 22. RAW264.7 cells received 600 V for 100 μsec. followed by 100 V for 10 msec.; U937 cells 400 V for 400 μsec. 22. For HDAC5 over-expression RAW264.7 cells were transfected with a pCMX-based vector encoding for HA-tagged mouse HDAC5 (pHDAC5; 8267 bp) kindly provided by Ronald Evans (Salk Institute, San Diego, CA, USA) 23. To determine NF-κB activation, RAW264.7 cells were transfected with an NF-κB reporter plasmid containing a single copy of an NF-κB response element linked to a luciferase sequence (pGL4.10; 4242 bp; Clontech, Mountain View, CA, USA). In RAW264.7 cells HDAC5 expression was knocked down by transfecting HDAC5-specific (siHDAC5) or control siRNA (siCtrl; Table​Table11).

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Manufacturer protocol

Download the product protocol from Qiagen for Mm_Hdac5_2 FlexiTube siRNA below.

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