PU.1 siRNA (m)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate transfection mixture for 5 min at room temperature. Incubate cells for 1–3 days at 37°C.

Protocol tips
Incubate transfection mixture for 5 min at room temperature. Incubate cells for 1–3 days at 37°C.

Publication protocol

Murine Nrf2 [31] and L-PGDS [18] were subcloned into pcDNA3.1(+) (Invitrogen), the fidelity of which was verified by sequencing. DNA was transfected using Lipofectamine 2000 and the manufacturer’s protocol (Invitrogen). Scrambled and siRNA specific for PU.1 was purchased and transfected per the manufacturer’s manual (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for PU.1 siRNA (m) below.

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