MEK-3 siRNA (m)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-50nM Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes. Add mixture to cells and incubate for 24-72hours.

Protocol tips
siRNA concentration-50nM Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes. Add mixture to cells and incubate for 24-72hours.

Publication protocol

For depletion of MKK3 in BMDMs, MEK-3 siRNA (m) (Santa Cruz, Cat# 35908) was used. Nonspecific siRNA scrambled duplex probes (sense, 5′-GCGCGCUUUGUAGGAUUCG-3′; antisense, 5′-CGAAUCCUACAAAGCGCGC-3′) were synthesized by Dharmacon Research Inc. as previously described. BMDMs (70% confluence) were transfected with the siRNAs (50nM) using the Mirus TransIT-TKO® reagent (Mirus, Madison, WI) according to the manufacturer’s manual for 48h. The experiments were performed after fresh media change.

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Manufacturer protocol

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