Stk11 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Concentration of siRNA- 0.5 µM. Incubate transfection mixture for 10 min at room temperature. Replace medium after four hours and incubate for additional 24 h.

Protocol tips
Concentration of siRNA- 0.5 µM. Incubate transfection mixture for 10 min at room temperature. Replace medium after four hours and incubate for additional 24 h.

Publication protocol

LKB1 siRNA (Stealth RNAi, Life Technologies) and control siRNA (Life Technologies) were used. The 3T3-L1 adipocytes or C2C12 myotubes cultured in 60-mm dishes (Becton Dickinson) were transfected with a 0.5 µM concentration of either siRNA by using the transfection reagent Lipofectamine 2000 (Life Technologies). Prior to transfection, 12.5 µl of siRNA and 5 µl of transfection reagent were diluted with 500 µl of serum-free medium in separate tubes at first, and then the diluted siRNA and Lipofectamine were mixed together. The mixtures were incubated for 10 min at room temperature and then added dropwise to each culture well. Four hours after transfection, the medium was exchanged for fresh medium. The cells were cultured for 24 h prior to the experiment. The efficiency of LKB1 silencing was monitored by both quantitative reverse transcription-PCR and Western blotting.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - 3T3-L1 Stk11 using Stk11 siRNA from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Stk11 siRNA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - 3T3-L1 Stk11 using Stk11 siRNA from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.