|NRF2-siRNA, 5′-UGAAAGCACAGCAGAAUUTT-3′ and control-siRNA, 5′-GAGCGGCCGAGCAACGUCUAU-3′
siRNA concentration- 50 nM
Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells for 6h.
Incubate cells for another 24h at 37°C and quantify.
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