Nrf2 siRNA (m)

Protocol tips

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	NRF2-siRNA, 5′-UGAAAGCACAGCAGAAUUTT-3′ and control-siRNA, 5′-GAGCGGCCGAGCAACGUCUAU-3′ siRNA concentration- 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells for 6h. Incubate cells for another 24h at 37°C and quantify.

Protocol tips
NRF2-siRNA, 5′-UGAAAGCACAGCAGAAUUTT-3′ and control-siRNA, 5′-GAGCGGCCGAGCAACGUCUAU-3′ siRNA concentration- 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells for 6h. Incubate cells for another 24h at 37°C and quantify.

Publication protocol

Nrf2 siRNAs were purchased from Santa Cruz Biotechnology Inc., (Dallas, TX, USA), NRF2-siRNA, 5′-UGAAAGCACAGCAGAAUUTT-3′ and control-siRNA, 5′-GAGCGGCCGAGCAACGUCUAU-3′. According to the manufacturer's protocol, the siRNAs (final siRNA concentration, 10 nM) were transfected into B16-F10 cells by using the Lipofectamine® RNAiMAX transfection reagent (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA). The cells were seeded in 6-well culture plates and incubated with Nrf2 siRNA at 50 nM for 6 h in serum-free OPTI-MEM media (Invitrogen; Thermo Fisher Scientific Inc.). Following incubation for 24 h at 37°C, the transfected cells were used for subsequent experiments

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Manufacturer protocol

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