NFE2L2 siRNA

Protocol tips

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	siRNA sequences: 5′-UGGAGCAAGACUUGGGCCACUUAAA-3′ and 5′ UUUAAGUGGCCCAAGUCUUGCUCCA-3′ Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 12h at 37°C.

Protocol tips
siRNA sequences: 5′-UGGAGCAAGACUUGGGCCACUUAAA-3′ and 5′ UUUAAGUGGCCCAAGUCUUGCUCCA-3′ Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 12h at 37°C.

Publication protocol

B16 cells were transfected with 50 nM Nrf2-annealed siRNA (Life Technologies) using Lipofectamine 2000 for 12 h according to the manufacturer's recommendations. The siRNA sequences targeted the following murine Nrf2 sequences: 5′-UGGAGCAAGACUUGGGCCACUUAAA-3′ and 5′ UUUAAGUGGCCCAAGUCUUGCUCCA-3′. Control experiments were performed using equivalent amounts of the corresponding sense oligonucleotides and scrambled oligonucleotides with the same base composition and a randomized sequence (5′-AUGGGCUAAAUCAUCCGCAAGAUGG-3′ and 5′-ACUGGCCAUUUCAGCUGAACCUUUG-3′).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for NFE2L2 siRNA below.

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