iBONi siRNA pool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration of 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. After 48h of transfection treat cells with climacostol (30 μg/ml) for 24h.

Protocol tips
siRNA concentration of 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. After 48h of transfection treat cells with climacostol (30 μg/ml) for 24h.

Publication protocol

According to the manufacturer’s protocol, iBONI siRNA Pool (a mix of 3 RNA duplex - small interfering RNAs) (Riboxx, Radebeul, Germany) targeting mouse p53 (gene ID 22059; Supplementary Table S2) was mixed to Lipofectamine RNAiMax transfection reagent (Life Technologies, Monza, Italy). iBONI siRNA Pool negative control (non-targeting siRNA; Supplementary Table S2) (Riboxx, Radebeul, Germany) was also used. The mix was added to cultured B16-F10 cells at a siRNA concentration of 50 nM. At 48 h after transfection, climacostol (24 h, 30 μg/ml) was then added before Western blotting and flow cytometry analyses.

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Manufacturer protocol

Download the product protocol from Riboxx for iBONi siRNA pool below.

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