ON-TARGETplus Mouse Lgals3 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siLGal3S3(1) J-041097-09 GAGAGAUACCCAUCGCUUU, siLGal3S3(2) J-041097-10 ACUUCAAGGUUGCGGUCAA, siLGal3S3(3) J-041097-11 ACAGUGAAACCCAACGCAA, siLGal3S3(4) J-041097-12 GGAUGAAGAACCUCCGGGA. Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for 1–3 days at 37°C.

Protocol tips
siLGal3S3(1) J-041097-09 GAGAGAUACCCAUCGCUUU, siLGal3S3(2) J-041097-10 ACUUCAAGGUUGCGGUCAA, siLGal3S3(3) J-041097-11 ACAGUGAAACCCAACGCAA, siLGal3S3(4) J-041097-12 GGAUGAAGAACCUCCGGGA. Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for 1–3 days at 37°C.

Publication protocol

Transfection of BV2 cells was carried out using Lipofectamine 2000 following the manufacturer’s recommendation (Life Technologies). Non-targeting control and galectin-3 siRNAs were obtained from Dharmacon. (SMART pool) siRNA sequence used: siLGal3S3(1) J-041097-09 GAGAGAUACCCAUCGCUUU, siLGal3S3(2) J-041097-10 ACUUCAAGGUUGCGGUCAA, siLGal3S3(3) J-041097-11 ACAGUGAAACCCAACGCAA, siLGal3S3(4) J-041097-12 GGAUGAAGAACCUCCGGGA.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Mouse Lgals3 siRNA below.

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