Tlr4 Mouse siRNA Oligo Duplex

siRNA / miRNA gene silencing Mouse - BV2 TLR4

Experiment
siRNA / miRNA gene silencing Mouse - BV2 TLR4
Product
Tlr4 Mouse siRNA Oligo Duplex from OriGene
Manufacturer
OriGene

Protocol tips

Protocol tips
siRNA concentration-10 nM.

Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells.

Incubate cells for another 48h at 37°C and quantify

Publication protocol

At 60% confluence, BV-2 cells were transfected with 10 nM TLR4 siRNA (Origene) or universal scrambled negative control siRNA (Origene) by using Lipofectamine RNAiMAX (Life Technologies). At 48 h after transfection, cells were used for various experiments. (Supplementary Fig. 4)

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Discussion

Discussion

1 year ago

Author:

Bv2 GAS5 gene silencing

Hello Iam a phd student in pharmacy and i want to know if this technology is suitable to knockout or silencing part of the gas5 gene in BV2 cells please

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Papers

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Manufacturer protocol

Download the product protocol from OriGene for Tlr4 Mouse siRNA Oligo Duplex below.

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