Protocol tips |
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siRNA concentration-10 nM. Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for another 48h at 37°C and quantify |
Tlr4 Mouse siRNA Oligo Duplex from OriGene has not yet been reviewed for this experiment
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Discussion
1 year ago
Author:
Hello Iam a phd student in pharmacy and i want to know if this technology is suitable to knockout or silencing part of the gas5 gene in BV2 cells please
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