Tlr4 Mouse siRNA Oligo Duplex
siRNA / miRNA gene silencing Mouse - BV2 TLR4
Protocol tips
| Protocol tips |
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| siRNA concentration-10 nM. Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for another 48h at 37°C and quantify |
Publication protocol
At 60% confluence, BV-2 cells were transfected with 10 nM TLR4 siRNA (Origene) or universal scrambled negative control siRNA (Origene) by using Lipofectamine RNAiMAX (Life Technologies). At 48 h after transfection, cells were used for various experiments. (Supplementary Fig. 4)Full paper Login or join for free to view the full paper.
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Discussion
Discussion
7 months ago
Author:
Bv2 GAS5 gene silencing
Hello Iam a phd student in pharmacy and i want to know if this technology is suitable to knockout or silencing part of the gas5 gene in BV2 cells please
Papers
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Manufacturer protocol
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