Timp2 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 0.5 × 10^5 cells per well in a 12-well plate.	siRNA TIMP-2 -100 pM TIMP-2 siRNA #1 (5′-GAAGGAGUAUCUAAUUGCATT-3′), TIMP-2 siRNA #2 (5′-UCCACAGACUUCAGCGAAU-3′), Mix diluted RNA and diluted siPORT™ NeoFX™ Transfection Agent; incubate 10 min at room temp, Incubate the transfected cells at 37°C for 48h.

Upstream tips
Seed 0.5 × 10^5 cells per well in a 12-well plate.
Protocol tips
siRNA TIMP-2 -100 pM TIMP-2 siRNA #1 (5′-GAAGGAGUAUCUAAUUGCATT-3′), TIMP-2 siRNA #2 (5′-UCCACAGACUUCAGCGAAU-3′), Mix diluted RNA and diluted siPORT™ NeoFX™ Transfection Agent; incubate 10 min at room temp, Incubate the transfected cells at 37°C for 48h.

Publication protocol

BV2 cells (0.5 × 105 cells per well in a 12-well plate) were transiently transfected with 100 pM of TIMP-2 small interfering RNA (siRNA) using Ambion siPORT™ NeoFX™ transfection reagent following the manufacturer’s protocols (Ambion). A scrambled control siRNA was used as a negative control. The siRNA sequences are as follows: TIMP-2 siRNA #1 (5′-GAAGGAGUAUCUAAUUGCATT-3′), TIMP-2 siRNA #2 (5′-UCCACAGACUUCAGCGAAU-3′), and Control siRNA (5′-AAUCGCAUAGCGUAUGCCGUU-3’). The cells were harvested 48 h after siRNA transfection, and the expression levels of TIMP-2 protein were measured by western blotting.

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