ON-TARGETplus Mouse Gpam siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA's concentration-25 nM. Sequence 5'-3' CCACAUACUGUCUCGUGAA GGUGAGGAGCAGCGAGAUU CGUACAUCGCCUCGGGCAA CUUAUCACCAGGACGGAAA Incubate HiPerFect Transfection Reagent to diluted siRNA for 10–15 min at RT. Incubate the cells with the transfection complexes under their normal growth conditions for 24 h

Protocol tips
siRNA's concentration-25 nM. Sequence 5'-3' CCACAUACUGUCUCGUGAA GGUGAGGAGCAGCGAGAUU CGUACAUCGCCUCGGGCAA CUUAUCACCAGGACGGAAA Incubate HiPerFect Transfection Reagent to diluted siRNA for 10–15 min at RT. Incubate the cells with the transfection complexes under their normal growth conditions for 24 h

Publication protocol

Prior to transplantation or hypoxia treatment, siRNAs specific for gpat1 or NT siRNA as a negative control were transfected to mESCs for 24 h with HiPerfect transfection reagent (QIAGEN) according to the manufacturer's description. Each transfected siRNA's concentration was 25 nM. The siRNA sequences are described in Supplementary Table S2.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Mouse Gpam siRNA below.

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