ON-TARGETplus Mouse Jun siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C.

Protocol tips
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C.

Publication protocol

RNAi was performed using transiently transfected small interfering RNA (siRNA) as described previously 20 using ON‐TARGET plus siRNAs (Thermo Scientific, http://www.thermoscientific.com): Nontargeting Control (NTC) (D‐001810‐10‐05), Jun (L‐043776‐00‐0005), Itgb1 (L‐040783‐01‐0005), Itgav (L‐046779‐01‐0005), and Fn1 (L‐043446‐01‐0005).

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Mouse Jun siRNA below.

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