Vps13a siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 2 x 10^5 cells and culture for 24 h at 37°C, 5% CO2.	Mix diluted RNA and diluted siPORT Amine agent and incubate for 10 min at room temp. Transfect cells with mixed transfection complexes and incubate for 72 h.

Upstream tips
Seed 2 x 10^5 cells and culture for 24 h at 37°C, 5% CO2.
Protocol tips
Mix diluted RNA and diluted siPORT Amine agent and incubate for 10 min at room temp. Transfect cells with mixed transfection complexes and incubate for 72 h.

Publication protocol

PC12 cells were seeded in 6 well plates on a density of 2 x 105 and cultured under standard culture conditions (37°C, 5% CO2) for 24 h. The cells were subsequently transfected for 72 h with validated siRNA for VPS13A (chorein) (ID#s235043, Ambion, Darmstadt, Germany) or with a negative control siRNA (ID#s4390843, Ambion) using siPORT amine transfection agent (Ambion) according to the manufacturer's protocol. The efficiency of silencing was checked by RT-PCR

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Vps13a siRNA below.

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