C/EBP β siRNA (r)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Dissolve siRNA duplexes in 20 mM KCl, 6 mM HEPES, pH7.5, and 0.2 mM MgCl2	Transfect cells at 37C in a CO2 incubator for 24 h at a final siRNA concentration of 1 μM,

Upstream tips
Dissolve siRNA duplexes in 20 mM KCl, 6 mM HEPES, pH7.5, and 0.2 mM MgCl2
Protocol tips
Transfect cells at 37C in a CO2 incubator for 24 h at a final siRNA concentration of 1 μM,

Publication protocol

The delivery of siRNA pools into primary microglial cells or BV2 cells was performed using lipofectin (Invitrogen). siRNA duplexes specific for the inhibition of C/EBP‐α and C/EBP‐β expression in human cells were obtained from Santa Cruz Biotechnology, Inc. The pooled siRNA duplexes were dissolved in buffer (20 mM KCl, 6 mM HEPES, pH7.5, and 0.2 mM MgCl2). Cell transfection was conducted for 24 h at a final siRNA concentration of 1 μM, followed by normal growth medium. Scrambled siRNA, a nontargeting 20–25 nt siRNA, was used as negative control.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for C/EBP β siRNA (r) below.

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