Gabpb1 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate transfection mixture at room temperature for 20 min. siRNA concentration- 50 nM. Incubate cells for 1–3 days at 37°C.

Protocol tips
Incubate transfection mixture at room temperature for 20 min. siRNA concentration- 50 nM. Incubate cells for 1–3 days at 37°C.

Publication protocol

Oligonucleotides directed against rat Nrf2 and control siRNA (Ambion, Austin, TX, USA) were transfected into SCs using Lipofectamine RNAiMAX (Invitrogen, Eugene, OR, USA), according to the manufacturer's protocol. Briefly, both Nrf2 siRNA and control siRNA were diluted with Opti-MEM medium and then, diluted Lipofectamine RNAiMax was added. The transfection mixture was incubated at room temperature for 20 min. When SCs reached 30–50% confluence, the culture medium was replaced with DMEM (without FBS) and the transfection mixture was added to each well. The final concentration of siRNA was 50 nM. After SCs transfected with Nrf2 siRNA and control siRNA were treated with EPS, the effect of Nrf2 knockdown on intracellular GSH levels was assessed.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gabpb1 siRNA below.

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