siGENOME Rat Nrp1 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Prepare transfection mixture in in serum-free medium and incubate for 20 min at room temperature. After 4 h transfection medium replace with complete medium. siRNA concentration- 100 nM Incubate cells at 37°C in 5% CO2 for 24–48 hours.

Protocol tips
Prepare transfection mixture in in serum-free medium and incubate for 20 min at room temperature. After 4 h transfection medium replace with complete medium. siRNA concentration- 100 nM Incubate cells at 37°C in 5% CO2 for 24–48 hours.

Publication protocol

SCs were transfected with siGENOME SMART pools for NRP1 or NRP2 (Dharmacon) consisting of 4 siRNA duplexes per gene to a final concentration of 100 nM. Transfection was performed according to the manufacturer’s protocol, using DharmaFECT 3. In short, transfection mix was prepared by mixing siRNA and DharmaFECT 3, both diluted in serum-free medium. After incubation for 20 min at room temperature, pre-warmed (37°C) medium containing 0.5% FCS was added. Culture medium was removed from the cells and replaced by transfection mix. After 4 h, transfection mix was removed and replaced by culture medium. Individual siRNAs from the SMART pools were tested at a concentration of 100 nM each. When NRP1 and NRP2 SMART pools were combined, each pool had a final concentration of 50 nM.

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Manufacturer protocol

Download the product protocol from Dharmacon for siGENOME Rat Nrp1 siRNA below.

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