HMG-1 siRNA (r)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate siRNA: Transfection reagent mixturefor 5hours at 37° C in a CO2 incubator. Add 1ml of DMEM with 20 % FBS after incubation. Incubate the cells for an additional 18hour.

Protocol tips
Incubate siRNA: Transfection reagent mixturefor 5hours at 37° C in a CO2 incubator. Add 1ml of DMEM with 20 % FBS after incubation. Incubate the cells for an additional 18hour.

Publication protocol

Control siRNA, human σ-1R siRNA (sc-42250), human Src siRNA (sc-29228), human NF-κB p65 siRNA (sc-29410), and rat HMGB1 siRNA (sc-270015) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Signal Silence® p44/42 MAPK (Erk1/2) siRNA was purchased from Cell Signaling (Danvers, MA, USA). The siRNAs were prepared according to the transfection protocol for cell cultures from Santa Cruz Biotechnology. Briefly, 1 ml of siRNA transfection reagent mixture (transfection reagent, sc-29528; transfection medium, sc-36868) was co-incubated with C6 cells for 5 h in a 5 % CO2 incubator at 37 °C, and an equal amount of DMEM with 20 % FBS was then added. An additional incubation was performed for 18 h, and the procedure for conditioned media was then performed.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for HMG-1 siRNA (r) below.

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