IGF-IRα/β siRNA (r)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration- 100 nM Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C.

Protocol tips
siRNA concentration- 100 nM Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C.

Publication protocol

The gene silencing IGF1R siRNA (IGF1R-siRNA) and non-specific control siRNA (NC-siRNA) were purchased from Stanta Cruz (Santa Cruz Biotechnology, USA). The transfection of siRNAs in RGC-5 cells was conducted with a Lipofectamine 2000 reagent according to manufacturer’s recommendation. After reaching 50% ~60% confluence, RGC-5 cells were transfected with IGF1R-siRNA (100 nM) or NC-siRNA (100 nM). The effect of siRNA on knocking down IGF1R protein, as well as other related proteins were examined by western blotting 48 hours after transfection.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for IGF-IRα/β siRNA (r) below.

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