Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
|
The protocol is optimized for 0.1–1 µg of total RNA. |
|
Protocol tips |
The protocol is optimized for 0.1–1 µg of total RNA. |
Publication protocol
The RNA-seq libraries were generated with TruSeq Stranded Total RNA with Ribo-Zero Gold Kit and the samples were sequenced as 100-bp single-end reads using a Hi-Seq 2000 instrument with biological triplicates. The RNA-seq analysis was performed as previously described, where the ribosomal reads, if any, were filtered out and the remaining reads were mapped to the hg19 transcriptome. Differential gene expression was calculated using the Deseq2 version 1.4.5 package in R 3.1.0 using the mean value of gene-wise dispersion estimates. To find significant differentially expressed genes, we used 0.01 for adjusted p-value and >1 log2 fold change.
Full paper
Login or
join for free to view the full paper.
Reviews
TruSeq Stranded Total RNA from Illumina has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Human - MCF-7 using TruSeq Stranded Total RNA from Illumina.
Paper title
RUNX1 contributes to higher-order chromatin organization and gene regulation in breast cancer cells
Videos
Check out videos that might be relevant for performing RNA sequencing Human - MCF-7 using TruSeq Stranded Total RNA from Illumina. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.