TruSeq Stranded Total RNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The protocol is optimized for 0.1–1 µg of total RNA.

Protocol tips
The protocol is optimized for 0.1–1 µg of total RNA.

Publication protocol

The RNA-seq libraries were generated with TruSeq Stranded Total RNA with Ribo-Zero Gold Kit and the samples were sequenced as 100-bp single-end reads using a Hi-Seq 2000 instrument with biological triplicates. The RNA-seq analysis was performed as previously described, where the ribosomal reads, if any, were filtered out and the remaining reads were mapped to the hg19 transcriptome. Differential gene expression was calculated using the Deseq2 version 1.4.5 package in R 3.1.0 using the mean value of gene-wise dispersion estimates. To find significant differentially expressed genes, we used 0.01 for adjusted p-value and >1 log2 fold change.

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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded Total RNA below.

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