KAPA Stranded RNA-Seq Kits with RiboErase (HMR)

Protocol tips

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	Total RNAs were assayed in both the whole transcriptome TempO-Seq assay (12 replicates each) and in RNA-Seq (6 replicates each), using ribosomal RNA depletion (KAPA Stranded RNA-Seq with RiboErase kit, Kapa cat # KK8483).

Protocol tips
Total RNAs were assayed in both the whole transcriptome TempO-Seq assay (12 replicates each) and in RNA-Seq (6 replicates each), using ribosomal RNA depletion (KAPA Stranded RNA-Seq with RiboErase kit, Kapa cat # KK8483).

Publication protocol

Total RNAs for TempO-Seq assay testing included the MAQC Universal Human Reference RNA (Agilent, cat#740000), MAQC Human Brain Reference RNA, and a selection of human tissue total RNAs (Thermo Fisher Scientific, cat # AM6050 and others). Synthetic RNAs for testing absolute sensitivity and fold change were the ERCC ExFold RNA Spike-In Mixes, obtained from Thermo Fisher Scientific (cat # 4456739). For the RNA-Seq cross-platform comparison, MCF-7 and MDA MB 231 cells were lysed according to the TempO-Seq protocol and RNA was purified using TRIzol (Thermo Fisher cat # 15596026). Total RNAs were assayed in both the whole transcriptome TempO-Seq assay (12 replicates each) and in RNA-Seq (6 replicates each), using ribosomal RNA depletion (KAPA Stranded RNA-Seq with RiboErase kit, Kapa cat # KK8483)

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Manufacturer protocol

Download the product protocol from Roche Lifesciences for KAPA Stranded RNA-Seq Kits with RiboErase (HMR) below.

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