TruSeq RNA Library Prep Kit v2

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Publication protocol

Total RNA was isolated from untreated and treated PANC-1 cells with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) followed by clean up with RNeasy Mini kit (Qiagen, Valencia, CA, USA), according to the manufacturer’s instructions. Quantity and quality of DNase treated RNA were determined using Agilent 2100 Bioanalyzer (RNA Nano 6000) and Nanodrop, respectively. A total of 16 RNA samples were sequenced [4 samples per condition × 4 conditions (untreated control, metformin, aspirin or a combination of metformin and aspirin)] using 500 ng of total RNA as input. Library preparation and RNA sequencing was carried out using the Illumina TruSeq™ RNA Sample Prep Kit v2 according to the manufacturer’s protocol (Illumina, San Diego, CA, USA). Samples were sequenced on the Illumina HiSeq 2500, 2 × 100 bp paired-end reads, to a minimum depth of 30 million reads per sample.

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Manufacturer protocol

Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.

Download manufacturer protocol

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