TruSeq RNA Library Prep Kit v2
RNA sequencing Mouse - ESCs (Embryonic Stem Cells)
Protocol tips
| Protocol tips |
|---|
| 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) |
Publication protocol
Total RNA (1 μg) was used for deep-sequencing library construction following the instructions of the TruSeq RNA sample preparation kit (Illumina). The final quality-ensured libraries were sequenced on a HiSeq 2000 sequencer generating 100 bp paired-end reads.Full paper Login or join for free to view the full paper.
Reviews
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Discussion
Discussion
5 years ago
Author:
Panagiotis Karagiannis
Using RNA from formalin fixed paraffin embedded tissue sections for sequencing
Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?
Papers
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Manufacturer protocol
Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.
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