TruSeq Stranded mRNA

RNA sequencing Mouse - Neuro 2a

Experiment

RNA sequencing Mouse - Neuro 2a

Product

TruSeq Stranded mRNA from Illumina

Manufacturer

Illumina

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The protocol is optimized for 0.1–1 µg of total RNA.

Protocol tips
The protocol is optimized for 0.1–1 µg of total RNA.

Publication protocol

Samples collected for RNA sequencing were prepped with TruSeq Stranded mRNA Sample Prep Kit (Illumina) and deep sequenced on the Illumina HiSeq platform. FASTQ files for each RNA sequencing library was mapped to the mouse mm10 genome by STAR using the parameters of ‘—outFilterMultimapNmax 1 –outFilterMismatchNmax 3'. The read count of each gene was performed by in-house Perl programmes and was normalized by edgeR.

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Discussion

5 years ago

Author: Panagiotis Karagiannis Greece

Using RNA from formalin fixed paraffin embedded tissue sections for sequencing

Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?

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Papers

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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

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