TruSeq Stranded mRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The protocol is optimized for 0.1–1 μg of total RNA.

Protocol tips
The protocol is optimized for 0.1–1 μg of total RNA.

Publication protocol

Live, cultured cells (Flp-In T-Rex 293 HEK cells, NIH/3T3 cells), intact, live Drosophila melanogaster embryos and sorted, methanol-fixed cells from dissected newborn mouse hindbrain and cerebellum were used for total RNA extraction with TRIZOL. Strand-specific cDNA libraries were generated according to the Illumina TruSeq protocol (TruSeq Stranded mRNA LT Sample Prep Kit, Illumina) using between 24 to 260 ng of total RNA input. The 1.8-pM libraries were sequenced on an Illumina NextSeq 500 System, using the High Output v2 Kit (150 cycles), single read: 150 bp, index read: 6 bp.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Mouse - 3T3-L1 using TruSeq Stranded mRNA from Illumina.

Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA sequencing Mouse - 3T3-L1 using TruSeq Stranded mRNA from Illumina. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.