TruSeq Stranded mRNA

Protocol tips

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	The protocol is optimized for 0.1–1 µg of total RNA.

Protocol tips
The protocol is optimized for 0.1–1 µg of total RNA.

Publication protocol

For the primary dataset, hepatocytes were isolated from a single rat liver, and total RNA was isolated from triplicate samples of uninfected, AdGFP-HBV- or AdGFP-infected PRHs at indicated time points for a total of 24 samples. The 0hr time point refers to PRHs that were isolated and purified, but not plated, while all other samples were plated and maintained for the indicated time. RNA was isolated from all samples following the total RNA protocol from the mirVana RNA isolation kit (Life Technologies, Carlsbad, CA). Total RNA was submitted to the Drexel University College of Medicine Center for Genomic Sciences (DUCOM CGS) for quality control, polyA selection, cDNA library preparation, and sequencing. The cDNA library was prepared using the TruSeq Stranded mRNA Prep Kit (Illumina), and was sequenced using the Illumina NextSeq 500 platform over two runs with 75 cycle High Output kits (ver. 1). One uninfected 24hr sample was lost during cDNA library creation, leaving a final total of 23 samples that were sequenced.

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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

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