TruSeq RNA Library Prep Kit v2

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Publication protocol

We used a Truseq RNA sample prep kit v2 (Illumina) to prepare a cDNA library for RNA-seq, following the manufacturer’s protocol. A total of 1.5 μg of RNA from each retinal sample was used to synthesize the cDNA library, which was used for 100 bp paired-end sequencing. We prepared six cDNA libraries (in triplicate) for cluster generation and sequencing. Each of them was indexed to allow for multiplex sequencing, and then applied to an Illumina flow cell for cluster generation. Clonal clusters were generated using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) on a cBot fluidics device according to the manufacturer’s protocol. After cluster generation, sequencing was performed on one lane of the Illumina Hiseq2500 device. The resulting sequence data were recorded in the FASTQ format. All RNA-seq data are available under the accession number DRA004746.

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Manufacturer protocol

Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.

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