TruSeq Stranded mRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The protocol is optimized for 0.1–1 µg of total RNA.

Protocol tips
The protocol is optimized for 0.1–1 µg of total RNA.

Publication protocol

RNA samples were processed by the high-throughput Genomics group at the Wellcome Trust Centre for Human Genetics. Samples were amplified by SMARTer (ClonTech, CA, US) due to the low quantities of RNA extracted from individual stellate ganglia. Library preparation used total RNA with the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Samples were sequenced using an Illumina Hi-Seq 4000 (Illumina, CA, USA), with samples repeated across two lanes to reduce technical error and increase read depth. The samples were sequenced with a paired end protocol (75 base pairs).

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Papers

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Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

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Videos

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