RIPA Buffer

Protocol tips

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	Cells were lysed in RIPA buffer containing 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 % sodium deoxycholate, 0.1 % sodium dodecyl sulfate (SDS), 1 % Nonidet P40, 2 mM EDTA, 50 mM NaF, 50 mM β-glycerophosphate, and 1 mM phenylmethylsulfonyl fluoride. Cell lysates were then passed through a 21-gauge needle followed by centrifugation at 4 °C for 30 min at 13,500g. The supernatant was used as a cell protein sample.

Protocol tips

Cells were lysed in RIPA buffer containing 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 % sodium deoxycholate, 0.1 % sodium dodecyl sulfate (SDS), 1 % Nonidet P40, 2 mM EDTA, 50 mM NaF, 50 mM β-glycerophosphate, and 1 mM phenylmethylsulfonyl fluoride. Cell lysates were then passed through a 21-gauge needle followed by centrifugation at 4 °C for 30 min at 13,500g. The supernatant was used as a cell protein sample.

Publication protocol

Cells were lysed in RIPA buffer containing 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 % sodium deoxycholate, 0.1 % sodium dodecyl sulfate (SDS), 1 % Nonidet P40, 2 mM EDTA, 50 mM NaF, 50 mM β-glycerophosphate, and 1 mM phenylmethylsulfonyl fluoride. Cell lysates were then passed through a 21-gauge needle followed by centrifugation at 4 °C for 30 min at 13,500g. The supernatant was used as a cell protein sample.

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