RIPA Lysis and Extraction Buffer

Protocol tips

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	The cells were rinsed with chilled PBS three times and subject to RIPA lysis (Thermo). To purify surface protein, the lysates were mixed with NeutrAvidin-agarose beads (Thermo) at 4°C overnight.

Protocol tips
The cells were rinsed with chilled PBS three times and subject to RIPA lysis (Thermo). To purify surface protein, the lysates were mixed with NeutrAvidin-agarose beads (Thermo) at 4°C overnight.

Publication protocol

The cells were rinsed with chilled PBS three times and subject to RIPA lysis (Thermo). To purify surface protein, the lysates were mixed with NeutrAvidin-agarose beads (Thermo) at 4°C overnight.

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