CelLytic™ M

Protocol tips

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	Cells growing in log phase were transfected with the indicated RNA at 50–75% confluence using TransIT-mRNA transfection kits (Mirus Bio, Madison, WI, USA). After 72 h, the cells were lysed, and proteins were extracted in CelLytic™ M Mammalian Cell Lysis/Extraction Reagent (Sigma-Aldrich, St. Louis, Missouri) supplemented freshly with 25 U/μL benzonase (EMD Millipore, Billerica, MA, USA), and cOmplete, Mini EDTA-free Protease Inhibitors (Roche Life Science, Mannheim, Germany) according to the manufacturer’s recommendations.

Protocol tips

Cells growing in log phase were transfected with the indicated RNA at 50–75% confluence using TransIT-mRNA transfection kits (Mirus Bio, Madison, WI, USA). After 72 h, the cells were lysed, and proteins were extracted in CelLytic™ M Mammalian Cell Lysis/Extraction Reagent (Sigma-Aldrich, St. Louis, Missouri) supplemented freshly with 25 U/μL benzonase (EMD Millipore, Billerica, MA, USA), and cOmplete, Mini EDTA-free Protease Inhibitors (Roche Life Science, Mannheim, Germany) according to the manufacturer’s recommendations.

Publication protocol

Cells growing in log phase were transfected with the indicated RNA at 50–75% confluence using TransIT-mRNA transfection kits (Mirus Bio, Madison, WI, USA). After 72 h, the cells were lysed, and proteins were extracted in CelLytic™ M Mammalian Cell Lysis/Extraction Reagent (Sigma-Aldrich, St. Louis, Missouri) supplemented freshly with 25 U/μL benzonase (EMD Millipore, Billerica, MA, USA), and cOmplete, Mini EDTA-free Protease Inhibitors (Roche Life Science, Mannheim, Germany) according to the manufacturer’s recommendations.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ M below.

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