pGL3-Basic Vector

Reporter gene assay luciferase - SW480 human colon cancer cell line

Experiment
Reporter gene assay luciferase - SW480 human colon cancer cell line
Product
pGL3-Basic Vector from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
Cut vector between KpnI-BglII fragments

Insert 0.3 kb and 0.17 kb DNA fragments

Publication protocol

In our previous study, the reporter plasmids, pGL3-0.3 and pGL3-0.17, in which the promoter regions −253/+47 and −122/+47 of the β4GalT4 gene relative to the transcriptional start site were inserted into the firefly luciferase reporter vector, pGL3-Basic (Promega, Madison, WI, USA), were constructed [10]. To establish the stable sensor cells having the luciferase gene under the control of the β4GalT4 gene promoters from SW480 cells, two reporter plasmids containing 0.3 kb and 0.17 kb promoter regions were prepared using pGL4.15[luc2p/Hygro] vector (Promega), which contains hygromycin-resistant gene. In brief, after the KpnI-BglII fragments were excised from pGL3-0.3 and pGL3-0.17, the 0.3 kb and 0.17 kb DNA fragments were inserted between KpnI and BglII sites of pGL4.15[luc2p/Hygro] vector to generate pGL4-0.3 and pGL4-0.17, respectively.

Full paper   Login or join for free to view the full paper.

Reviews

pGL3-Basic Vector from Promega has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: J.Han China

Which transfection reagent would work better on stem cells?

I am planning on running reporter assays on stem cells and I would like to know which transfection reagent would work better?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay luciferase - SW480 human colon cancer cell line using pGL3-Basic Vector from Promega.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Promega for pGL3-Basic Vector below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay luciferase - SW480 human colon cancer cell line using pGL3-Basic Vector from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms