CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - ME epithelial tissue

Experiment

Protein isolation Tissue - ME epithelial tissue

Product

CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich

Manufacturer

Sigma-Aldrich

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The tissues were homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C.	Protein concentration was determined using the DC Protein Assay kit (Bio-Rad).

Protocol tips
The tissues were homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C.
Downstream tips
Protein concentration was determined using the DC Protein Assay kit (Bio-Rad).

Publication protocol

The tissues were homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C. Protein concentration was determined using the DC Protein Assay kit (Bio-Rad).

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Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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