NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Protocol tips

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	Cells were grown in 6-well plates until subconfluence and then exposed to NTHi lysates for the times indicated. Cells were washed with phosphate-buffered saline and then fractionated using nuclear and cytoplasmic extraction reagents (NE-PER; Pierce Biotechnology) according to the manufacturer’s recommendations.

Protocol tips
Cells were grown in 6-well plates until subconfluence and then exposed to NTHi lysates for the times indicated. Cells were washed with phosphate-buffered saline and then fractionated using nuclear and cytoplasmic extraction reagents (NE-PER; Pierce Biotechnology) according to the manufacturer’s recommendations.

Publication protocol

Cells were grown in 6-well plates until subconfluence and then exposed to NTHi lysates for the times indicated. Cells were washed with phosphate-buffered saline and then fractionated using nuclear and cytoplasmic extraction reagents (NE-PER; Pierce Biotechnology) according to the manufacturer’s recommendations.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for NE-PER™ Nuclear and Cytoplasmic Extraction Reagents below.

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