NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Protein isolation Tissue - ME epithelial tissue

Experiment
Protein isolation Tissue - ME epithelial tissue
Product
NE-PER™ Nuclear and Cytoplasmic Extraction Reagents from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Cells were grown in 6-well plates until subconfluence and then exposed to NTHi lysates for the times indicated. Cells were washed with phosphate-buffered saline and then fractionated using nuclear and cytoplasmic extraction reagents (NE-PER; Pierce Biotechnology) according to the manufacturer’s recommendations.

Publication protocol

Cells were grown in 6-well plates until subconfluence and then exposed to NTHi lysates for the times indicated. Cells were washed with phosphate-buffered saline and then fractionated using nuclear and cytoplasmic extraction reagents (NE-PER; Pierce Biotechnology) according to the manufacturer’s recommendations.

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Discussion

Discussion

1 year ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - ME epithelial tissue using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents from Thermo Fisher Scientific.

Paper title
Middle Ear Response of Muc5ac and Muc5b Mucins to Nontypeable Haemophilus influenzae.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for NE-PER™ Nuclear and Cytoplasmic Extraction Reagents below.

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