CelLytic™ MT Cell Lysis Reagent

Protocol tips

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	- The frozen tissues are homogenized in CelLytic™ MT cell lysis reagent by Precellys 24 beads homogenizer.

Protocol tips
- The frozen tissues are homogenized in CelLytic™ MT cell lysis reagent by Precellys 24 beads homogenizer.

Publication protocol

We conducted all mouse experiments in accordance with experimental protocols that were approved by the Animal Care and Use Committee of the Tohoku University Graduate School of Medicine (2013-461), which was granted by the Guide for Care and Use of Laboratory Animals published by the US National Institutes of Health. C57/BL6 mice were purchased from Charles River Laboratories Japan, Inc. (Kanagawa, Japan). C57/BL6 mice were administrated either atorvastatin (10 mg/kg per day), pravastatin (50 mg/kg per day), or placebo by gavage every day for 1 week. After the 1-week treatment, the animals were anaesthetized with isofluorane and perfused with ice-cold PBS. Immediately after dissection, the aorta, heart, liver, and skeletal muscles were frozen by liquid nitric oxide. The frozen tissues were homogenized in CelLytic™ MT cell lysis reagent (Sigma, C3228) by Precellys 24 beads homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France). The homogenates were centrifuged and the supernatants were used for western blot analysis.

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Manufacturer protocol

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