CelLytic™ MT Cell Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- The tissues are homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C.

Protocol tips
- The tissues are homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C.

Publication protocol

Total protein extracted from the ME epithelial cells and lungs of two-months-old wild-type, Nischedsn/edsn and Itga5tm1Hyn/+; Nischedsn/edsn mice were used for the western blot analysis. Each middle ear sample consisted of combined epithelial cells scooped out of both ears of one mouse. Each lung sample consisted of whole lung tissue from one mouse. Either three or four biological replicates were performed for each antibody. The tissues were homogenised in CelLytic MT Cell Lysis Reagent (Sigma C3228), protease inhibitors, phosphatase inhibitors and vanadate and centrifuged at 4°C. Protein concentration was determined using the DC Protein Assay kit (Bio-Rad).

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Manufacturer protocol

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