T-PER™ Tissue Protein Extraction Reagent

Protein isolation Tissue - Mouse lung tissue

Experiment

Protein isolation Tissue - Mouse lung tissue

Product

T-PER™ Tissue Protein Extraction Reagent from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- T-PER tissue extraction reagent containing Halt protease inhibitor mixture (Pierce Technology) at a final concentration of 1× is added at 20 ml/g of tissue, and samples are homogenized on ice. Tissue debris is removed by centrifugation for 5 min at 10,000 rpm.

Protocol tips
- T-PER tissue extraction reagent containing Halt protease inhibitor mixture (Pierce Technology) at a final concentration of 1× is added at 20 ml/g of tissue, and samples are homogenized on ice. Tissue debris is removed by centrifugation for 5 min at 10,000 rpm.

Publication protocol

Frozen mouse lungs were homogenized in the protease and phosphatase inhibitor cocktail, T-PER Tissue Protein Extraction Reagent (Thermo Scientific, Rockford, IL), as described.

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Reviews

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Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse lung tissue using T-PER™ Tissue Protein Extraction Reagent from Thermo Fisher Scientific.

Paper title

Leukocyte-specific protein 1 regulates neutrophil recruitment in acute lung inflammation

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for T-PER™ Tissue Protein Extraction Reagent below.

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Videos

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