Cell Lysis Buffer (10X)

Protein isolation Tissue - Mouse lung tissue

Experiment
Protein isolation Tissue - Mouse lung tissue
Product
Cell Lysis Buffer (10X) from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
- The tissue is lysed in cell lysis buffer (Cell Signaling) supplemented with complete EDTA-free protease inhibitors, PhosSTOP phosphatase inhibitors, 1 mM PMSF and 50 mM NaF.

Publication protocol

Whole lungs from E14.5 mice or caudal lobes from 10-week-old mice were lysed in cell lysis buffer (Cell Signaling) supplemented with complete EDTA-free protease inhibitors (Roche), PhosSTOP phosphatase inhibitors (Roche), 1 mM PMSF (Sigma-Aldrich) and 50 mM NaF (Sigma-Aldrich). Subcellular fractionation to isolate the membrane and cytoplasmic fraction was performed using a Plasma membrane Protein Extraction Kit (Abcam) following the manufacturer's instructions. Protein concentration was assessed by Bradford assay (Sigma-Aldrich) following the manufacturer's instructions.

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Reviews

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Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse lung tissue using Cell Lysis Buffer (10X) from Cell Signaling Technology.

Paper title
Heterozygous mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair
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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Cell Lysis Buffer (10X) below.

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