CelLytic™ NuCLEAR™ Extraction Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- First, cardiac tissue is homogenized in a hypotonic buffer (NXTRACT; Sigma) supplemented with a cocktail of protease and phosphatase inhibitors, according to recommended brand protocols (cOmplete, and PhosSTOP, Roche). - Then, nuclear protein extracts were separated from the homogenates using the NXTRACT CelLytic NuCLEAR Extraction Kit.

Protocol tips
- First, cardiac tissue is homogenized in a hypotonic buffer (NXTRACT; Sigma) supplemented with a cocktail of protease and phosphatase inhibitors, according to recommended brand protocols (cOmplete, and PhosSTOP, Roche). - Then, nuclear protein extracts were separated from the homogenates using the NXTRACT CelLytic NuCLEAR Extraction Kit.

Publication protocol

Protein expression was measured by performing Western blot analysis on heart extracts. Cardiac tissue was homogenized in a hypotonic buffer (NXTRACT; Sigma) supplemented with a cocktail of protease and phosphatase inhibitors, according to recommended brand protocols (cOmplete, and PhosSTOP, Roche). Nuclear protein extracts were separated from the homogenates using the NXTRACT CelLytic NuCLEAR Extraction Kit (Sigma). Immunodetection of protein expression was performed according to standard Western blotting protocols on nitrocellulose membrane. Values were expressed (in arbitrary units) as protein levels detected and normalized to the housekeeping protein GAPDH.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse cardiac tissue using CelLytic™ NuCLEAR™ Extraction Kit from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ NuCLEAR™ Extraction Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Tissue - Mouse cardiac tissue using CelLytic™ NuCLEAR™ Extraction Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.