|- First, cardiac tissue is homogenized in a hypotonic buffer (NXTRACT; Sigma) supplemented with a cocktail of protease and phosphatase inhibitors, according to recommended brand protocols (cOmplete, and PhosSTOP, Roche).
- Then, nuclear protein extracts were separated from the homogenates using the NXTRACT CelLytic NuCLEAR Extraction Kit.
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2 years ago
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
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