CelLytic™ NuCLEAR™ Extraction Kit

Protein isolation Tissue - Mouse cardiac tissue

Experiment
Protein isolation Tissue - Mouse cardiac tissue
Product
CelLytic™ NuCLEAR™ Extraction Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- First, cardiac tissue is homogenized in a hypotonic buffer (NXTRACT; Sigma) supplemented with a cocktail of protease and phosphatase inhibitors, according to recommended brand protocols (cOmplete, and PhosSTOP, Roche).

- Then, nuclear protein extracts were separated from the homogenates using the NXTRACT CelLytic NuCLEAR Extraction Kit.

Publication protocol

Protein expression was measured by performing Western blot analysis on heart extracts. Cardiac tissue was homogenized in a hypotonic buffer (NXTRACT; Sigma) supplemented with a cocktail of protease and phosphatase inhibitors, according to recommended brand protocols (cOmplete, and PhosSTOP, Roche). Nuclear protein extracts were separated from the homogenates using the NXTRACT CelLytic NuCLEAR Extraction Kit (Sigma). Immunodetection of protein expression was performed according to standard Western blotting protocols on nitrocellulose membrane. Values were expressed (in arbitrary units) as protein levels detected and normalized to the housekeeping protein GAPDH.

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Discussion

Discussion

2 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse cardiac tissue using CelLytic™ NuCLEAR™ Extraction Kit from Sigma-Aldrich.

Paper title
Impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ NuCLEAR™ Extraction Kit below.

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