RIPA Lysis Buffer (ProteinSimple)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Instructions are followed according to the manufacturer's protocol.

Protocol tips
- Instructions are followed according to the manufacturer's protocol.

Publication protocol

Total proteins were extracted and purified using protein extraction reagents (RIPA Lysis Buffer, 040-483; ProteinSimple) for zebrafish and mouse ventricular tissues (n = 4 per group). The equivalent of 50 μg of protein from each sample was separated by 11% gel [for 10 ml of gel: 3.85 ml of deionized water, 3.53 ml of 30% acrylamide, 2.6 ml of tris-HCl (pH 8.8), 50 μl of ammonium persulfate, and 10 μl of tetramethylethylenediamine; all from Bio-Rad Laboratories] and then transferred onto a polyvinylidene fluoride (PVDF) membrane (BioTrace PVDF Transfer Membrane, 66594).

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Manufacturer protocol

Download the product protocol from ProteinSimple for RIPA Lysis Buffer (ProteinSimple) below.

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