CelLytic™ MT Cell Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cut tissue is resuspended in 3 volumes of CelLytic Buffer plus 10% protease inhibitor mixture and Halt phosphatase inhibitor mixture and incubated on ice for 30. - After the the livers are lysed using a Qiagen Tissue Lyser LT and then centrifuged at 100,000 × g at 4 °C.

Protocol tips
- Cut tissue is resuspended in 3 volumes of CelLytic Buffer plus 10% protease inhibitor mixture and Halt phosphatase inhibitor mixture and incubated on ice for 30. - After the the livers are lysed using a Qiagen Tissue Lyser LT and then centrifuged at 100,000 × g at 4 °C.

Publication protocol

Mouse tissues were flash frozen in liquid nitrogen during harvesting and stored at −80 °C. For gel electrophoresis, 50–100 mg of cut tissue was then resuspended in 3 volumes of CelLytic Buffer (Sigma C3228) plus 10% protease inhibitor mixture (P2714-1BTL; Sigma) and Halt phosphatase inhibitor mixture (PI78420; Fisher), then incubated on ice for 30 min followed by lysing the livers using a Qiagen Tissue Lyser LT (Qiagen), and then centrifuged at 100,000 × g at 4 °C.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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