Tissue Extraction Reagent I

Protein isolation Tissue - Mouse liver tissue

Experiment
Protein isolation Tissue - Mouse liver tissue
Product
Tissue Extraction Reagent I from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Total protein was isolated from mouse liver using Tissue Extraction Reagent I supplemented with cOmplete protease inhibitor cocktail.

Publication protocol

Protein levels for candidate genes were determined by quantitative western blot. Each experiment was assessed on a single western blot to ensure identical analysis conditions between treated and control animals. Total protein was isolated from mouse liver using Tissue Extraction Reagent I (Life Technologies, Burlington, ON) supplemented with cOmplete protease inhibitor cocktail (Roche, Laval, QC). Protein extract, diluted 1/10 and 1/20 with 1XPBS, was quantified by Bradford assay and diluted to a final concentration of 10 µg/µL. A total of 65 µg protein, was loaded into each well of a Novex 4–12% Bis-Tris midi-gel system to ensure sufficient material would be available for the detection of low abundance targets.

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Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse liver tissue using Tissue Extraction Reagent I from Thermo Fisher Scientific.

Paper title
Identification of Reference Proteins for Western Blot Analyses in Mouse Model Systems of 2,3,7,8-Tetrachlorodibenzo-P-Dioxin (TCDD) Toxicity
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Tissue Extraction Reagent I below.

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Videos

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