NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Protein isolation Tissue - Mouse liver tissue

Experiment
Protein isolation Tissue - Mouse liver tissue
Product
NE-PER™ Nuclear and Cytoplasmic Extraction Reagents from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For nuclear protein extraction, the liver samples are collected 60 min after reperfusion and immediately homogenized with a prechilled Dounce homogenizer. Nuclear protein is extracted with NE-PER nuclear and cytoplasmic extraction reagents.

Publication protocol

Whole cell protein was extracted from liver tissue with T-PER tissue protein extraction reagent (Pierce Biotechnology, Rockford, IL, USA). For nuclear protein extraction, the liver samples were collected 60 min after reperfusion and immediately homogenized with a prechilled Dounce homogenizer (Wheaton Industries, Millville, NJ, USA). Nuclear protein was extracted with NE-PER nuclear and cytoplasmic extraction reagents (Pierce Biotechnology). Protein concentration was determined using the Bradford method.

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Discussion

Discussion

2 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse liver tissue using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents from Thermo Fisher Scientific.

Paper title
SPA0355 attenuates ischemia/reperfusion-induced liver injury in mice
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for NE-PER™ Nuclear and Cytoplasmic Extraction Reagents below.

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