CelLytic™ MT Cell Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Prostatic tissue is homogenized in tissue lysis/extraction reagent containing protease inhibitor cocktail using a homogenizer (T10 Basic Ultra Turrax; IKA Works, Staufen, Germany). Homogenates are centrifuged at 15,500 g for 20 min at 4 °C.

Protocol tips
- Prostatic tissue is homogenized in tissue lysis/extraction reagent containing protease inhibitor cocktail using a homogenizer (T10 Basic Ultra Turrax; IKA Works, Staufen, Germany). Homogenates are centrifuged at 15,500 g for 20 min at 4 °C.

Publication protocol

Prostatic tissue was homogenized (1/10 w/v) in tissue lysis/extraction reagent (C3228; Sigma-Aldrich) contain- ing protease inhibitor cocktail (Ref 11 836 153 001; Roche, Mannheim, Germany) using a homogenizer (T10 Basic Ultra Turrax; IKA Works, Staufen, Germany). Homoge- nates were centrifuged at 15,500 g for 20 min at 4 °C. Total protein concentrations in the supernatant fractions were measured using Bradford reagent (No. 500–0006; Bio-Rad Laboratories, Inc., Hercules, CA, USA) as de- scribed previously.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

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