CelLytic™ MT Cell Lysis Reagent

Protein isolation Tissue - Mouse prostate tissue

Experiment
Protein isolation Tissue - Mouse prostate tissue
Product
CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Prostatic tissue is homogenized in tissue lysis/extraction reagent containing protease inhibitor cocktail using a homogenizer (T10 Basic Ultra Turrax; IKA Works, Staufen, Germany). Homogenates are centrifuged at 15,500 g for 20 min at 4 °C.

Publication protocol

Prostatic tissue was homogenized (1/10 w/v) in tissue lysis/extraction reagent (C3228; Sigma-Aldrich) contain- ing protease inhibitor cocktail (Ref 11 836 153 001; Roche, Mannheim, Germany) using a homogenizer (T10 Basic Ultra Turrax; IKA Works, Staufen, Germany). Homoge- nates were centrifuged at 15,500 g for 20 min at 4 °C. Total protein concentrations in the supernatant fractions were measured using Bradford reagent (No. 500–0006; Bio-Rad Laboratories, Inc., Hercules, CA, USA) as de- scribed previously.

Full paper   Login or join for free to view the full paper.

Reviews

CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse prostate tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.

Paper title
Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Tissue - Mouse prostate tissue using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms