T-PER™ Tissue Protein Extraction Reagent
Protein isolation Tissue - Mouse prostate tissue
Publication protocol
Prostate tissues were extracted by using the T-PER protein extraction reagent (Pierce). An equal amount of prostate tissue or total cell lysates was subjected to SDS-PAGE, followed by Western blot analysis using anti-AR(441), anti-actin (C2), anti-AR (N20; Santa Cruz Biotechnology), anti-TGF-β, anti-Smad3, anti-p-Smad3, anti-Smad1, anti-p-Smad1/5 (Cell Signaling), and anti-AR3.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
When should I be harvesting the tissue before using it for the most optimal results?
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
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Manufacturer protocol
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