T-PER™ Tissue Protein Extraction Reagent

Protein isolation Tissue - Mouse prostate tissue

Experiment
Protein isolation Tissue - Mouse prostate tissue
Product
T-PER™ Tissue Protein Extraction Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Instructions were followed according to the manufacturer's protocol.

Publication protocol

Prostate tissues were extracted by using the T-PER protein extraction reagent (Pierce). An equal amount of prostate tissue or total cell lysates was subjected to SDS-PAGE, followed by Western blot analysis using anti-AR(441), anti-actin (C2), anti-AR (N20; Santa Cruz Biotechnology), anti-TGF-β, anti-Smad3, anti-p-Smad3, anti-Smad1, anti-p-Smad1/5 (Cell Signaling), and anti-AR3.

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Reviews

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Discussion

Discussion

5 years ago

Author: Spain

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Tissue - Mouse prostate tissue using T-PER™ Tissue Protein Extraction Reagent from Thermo Fisher Scientific.

Paper title
Androgen Receptor Splice Variant AR3 Promotes Prostate Cancer via Modulating Expression of Autocrine/Paracrine Factors
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for T-PER™ Tissue Protein Extraction Reagent below.

Download PDF Download manufacturer protocol

Videos

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